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Single molecule studies of molecular structure and function in vitro and in vivo

时间:2017-03-30 19:16:00作者:来源:



报告题目:Single molecule studies of molecular structure and functionin vitroandin vivo

:Dr. Peiwu Qin,University of California,Berkeley

报告时间:2017331日(星期五)10:00-12:00

报告地点:基础科学大楼B253

报告摘要:

Single molecule imaging has been used extensively to study molecular conformation, dynamics, and function. Here, I will demonstrate the application of single molecule imaging in ribosome dynamicsin vitroand chromosome targetingin vivo. During protein synthesis, mRNA and tRNA are moved through the ribosome by the process of translocation. The small diameter of the mRNA entrance tunnel only permits unstructured mRNA to pass through. However, there are structured elements within mRNA that present a barrier for translocation that must be unwound. We discovered a new global conformational state of the ribosome by single molecule Förster resonance energy transfer and small angle X-ray scattering. In the presence ofdnaXhairpin, the ribosomal subunits are driven into a hyper-rotated state and L1 stalk is predominantly in an open conformation.Imaging chromatin dynamics are mostly applicable to highly repetitive regions by CRISPR/Cas9 previously. We designed single-guide RNAs integrated with up to 16xMS2 binding motifs to enable robust fluorescent signal amplification. These engineered sgRNAs enable multicolor labeling with low-repeat-containing regions using a single sgRNA and non-repetitive regions with as few as four unique sgRNAs. We achieve tracking of native chromosome loci throughout the cell cycle and determine differential positioning of transcriptional active and inactive regions in the nucleus.

介:秦培武博士,2002年本科毕业于东北农业大学生物工程系;2005年硕士毕业于中国科学院遗传与发育生物学研究所,从事斑马鱼胚胎发育研究;2008硕士毕业于美国东北大学物理化学专业,研究药物的分子机理;2013年博士毕业于密苏里大学,用NMR技术研究蛋白的动态结构和单分子FRET研究RNA结构对核糖体动态的影响。2013年至今在加州大学伯克利从事博士后研究,主要用超分辨率光学成像的方法研究端粒的结构和光片照片显微镜对非重复区Cas9标记的染色体。研究工作在Cell、Nature Communications、EMBO Report等期刊上发表十余篇论文。